SiRNA Validation Data

siRNA ID:

s7186

Gene ID:

3455

Transcript(s):

NM_000874.4 NM_001289125.1 NM_001289126.1 NM_001289128.1 NM_207584.2 NM_207585.2

Validation Results:

13%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00174198_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.