SiRNA Validation Data



Gene ID:



NM_000309.3 NM_001122764.1 XM_005245291.3 XM_005245295.3 XM_006711402.2 XM_006711403.2 XM_006711404.3 XM_006711406.2 XM_011509663.2 XM_011509664.1 XM_011509665.2 XM_011509666.2 XM_011509667.2 XM_011509668.2 XM_011509669.1 XM_011509670.2 XM_011509671.1 XM_011509672.2 XM_011509673.2 XM_011509674.2 XM_011509675.2 XM_011509676.1 XM_011509677.1 XM_011509678.1 XM_011509679.1 XM_011509681.1 XM_011509682.1 XM_017001559.1 XM_017001560.1 XM_017001561.1 XM_017001562.1 XM_017001563.1 XM_017001565.1 XM_017001566.1 XM_017001567.1 XM_017001568.1 XM_017001569.1 XM_017001570.1 XM_017001571.1

Validation Results:

18%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00609392_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.