SiRNA Validation Data

siRNA ID:

104969

Gene ID:

142679

Transcript(s):

NM_001142314.1 NM_001321519.1 NM_080876.3 NR_135688.1

Validation Results:

13%(+/-3% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00369901_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.