SiRNA Validation Data



Gene ID:



NM_001227.4 NM_001267056.1 NM_001267057.1 NM_001320911.1 NM_033338.5 NM_033339.4 NM_033340.3 XM_006718017.3 XM_017016763.1 XM_017016764.1

Validation Results:

17%(+/-8% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00169152_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.