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SiRNA Validation Data

siRNA ID:

s10048

Gene ID:

5073

Transcript(s):

NM_001134477.2 NM_001242992.1 NM_002582.3 XM_011522510.2 XM_011522511.2 XM_011522513.2 XM_011522514.1 XM_017023258.1 XM_017023259.1 XM_017023260.1

Validation Results:

17%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00377733_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer^® Select siRNA or 5 nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.