SiRNA Validation Data



Gene ID:



NM_001130020.1 NM_001130021.1 NM_005177.3 XM_005257459.1 XM_005257461.1 XM_005257463.2 XM_011524908.1 XM_017024766.1 XM_017024767.1 XM_017024768.1 XM_017024769.1 XM_017024770.1 XM_017024771.1 XM_017024772.1 XM_017024773.1 XM_017024774.1

Validation Results:

15%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00193110_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.