SiRNA Validation Data

siRNA ID:

s35233

Gene ID:

65125

Transcript(s):

NM_001184985.1 NM_014823.2 NM_018979.3 NM_213655.4 XM_006719003.2 XM_011520997.2 XM_011520998.2 XM_011520999.2 XM_011521000.2 XM_011521001.2 XM_011521002.2 XM_011521003.2 XM_011521004.2 XM_011521005.2 XM_011521006.2 XM_011521007.2 XM_011521008.2 XM_011521009.2 XM_017019834.1 XM_017019835.1 XM_017019836.1 XM_017019837.1 XM_017019838.1 XM_017019839.1

Validation Results:

9%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00219183_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.