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SiRNA Validation Data

siRNA ID:

s8759

Gene ID:

4258

Transcript(s):

NM_001204366.1 NM_001204367.1 NM_002413.4 XM_006714221.2 XM_011531972.2 XM_017008211.1 XM_017008212.1 XM_017008213.1 XM_017008214.1

Validation Results:

1%(+/-0% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00182064_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer^® Select siRNA or 5nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan^® Gene Expression Cells-to-CT^™ Kit and appropriate TaqMan^® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.