Thermo Fisher Scientific
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Search all TaqMan™ Assays for mRNA and long
noncoding RNA analysis, including endogenous control and
marker/reporter gene products.
TaqMan™ Noncoding RNA Assays can detect and
quantify long (>200 bases) noncoding RNA (ncRNA) transcripts. Our
collection covers long ncRNA transcripts from sources including
NCBI RefSeq NR, RNAdb, and content on Invitrogen™
NCode™ Non-coding RNA Arrays.
Marker & Reporter assays can be
used to verify and quantify reporter gene products from
expression vectors that have been introduced into cells or
animals, such as lacZ or GFP.
Control Assays target commonly
used endogenous control or reference RNAs. These Control Assays
are typically used for normalization for relative gene expression
TaqMan™ Gene Expression Fusion Assays
are designed to detect fusion transcripts using real-time PCR.
Our collection covers gene fusion transcripts from sources
including NCBI Genbank and content on Oncomine™ products.
Search all TaqMan™ SNP Genotyping Assays,
including Drug Metabolism Genotyping Assays, and assays that are
functionally tested or validated.
These assays are specifically
designed to detect polymorphisms in 221 genes that code for
various drug metabolism enzymes and drug transporters. Assays are
tested using gDNA from 45 individuals from four ethnicities. The
minor allele frequency for each population is available, but rare
and mutant alleles may not be present in the test DNA.
Search all TaqMan™ Copy Number Assays and required
TaqMan™ Copy Number Reference Assays.
Marker & Reporter assays are
designed to detect the copy number of marker and reporter genes
from expression vectors that have been introduced into cells or
animals, such as the lacZ reporter gene or antibiotic genes.
Silencer® Select siRNAs incorporate the
latest improvements in siRNA design, off-target effect prediction
algorithms, and chemical modifications to yield siRNA with
unrivaled efficacy, potency, and specificity. They are
recommended for in vitro RNAi experiments.
Ambion® In Vivo siRNAs are designed using the Silencer®
Select algorithm and incorporate chemical modifications that help
provide superior serum stability for in vivo delivery.
Stealth RNAi siRNAs uses their
proprietary chemical modifications for restricting off-target
effects and their effective algorithm for providing efficient
knockdown. They are recommended for completing in vitro RNAi
studies that were started using Stealth siRNAs. For new studies,
we recommend Ambion Silencer Select siRNAs.
Silencer® siRNAs are designed using our
first-generation algorithm and do not include any chemical
modifications. They are recommended for completing in
vitro RNAi studies that were started using Silencer®
siRNA. For new studies, we recommend Silencer®
TrueGuide Modified Synthetic crRNAs are hybridized with TrueGuide tracrRNA to form a gRNA complex that directs Cas9 to the editing site.
The efficiency of the TrueGuide 2 piece gRNA system is enhanced by the addition of 2’O-meythl analogs and 3’ phosphorothioate internucleotide linkages
that enhance editing efficiency by increasing binding to the target site and inhibiting nuclease degradation, respectively.
Note: for this format requires the purchase of separate TrueGuide™ crRNA and TrueGuide™ tracrRNA products, which are annealed to generate a crRNA:tracrRNA duplex prior to delivery into cells.
Be sure to add the TrueGuide tracrRNA to cart when ordering TrueGuide™ crRNA.
TrueGuide Modified Synthetic sgRNAs are 1 piece, chemically synthesized, 100-mer gRNAs that incorporate 2’O-meythl analogs and 3’ phosphorothioate
internucleotide linkages in the terminal three nucleotides on the on both the 5’ and 3’ ends of the gRNA.
These modifications enhance editing efficiency by increasing binding to the target site and inhibiting nuclease degradation, respectively.
TrueGuide Synthetic sgRNAs are 1 piece, chemically synthesized, 100-mer gRNAs for high efficiency CRISPR editing.
LentiArray sgRNA are sgRNAs pre-packaged as ready to use lentiviral
particles. LentiArray sgRNAs provide the versatility to apply CRISPR
based editing to a wide variety cell lines including hard to transfect cell lines.
TaqMan™ Advanced miRNA Assays,
based on a universal RT approach, provide even greater sensitivity
in biological fluids and proven TaqMan™ specificity.
TaqMan™ MicroRNA Assays, based
on the specific stem-loop primer approach, are the gold standard
for sensitivity and specificity.
The miRNA control assays are unique to each assay chemistry type
(universal RT or miRNA- specific stem loop RT) and cannot be used
with the other assay chemistry.