104699
NM_001101669.1
NM_003866.2
XM_005263323.3
XM_005263325.3
XM_011532391.2
XM_011532392.2
XM_017008794.1
XM_017008795.1
XM_017008796.1
XM_017008797.1
XM_017008798.1
7%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00182580_m1]
HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.