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SiRNA Validation Data

siRNA ID:

111216

Gene ID:

5833

Transcript(s):

NM_001184917.2 NM_001256433.2 NM_001256434.2 NM_001256435.2 NM_001282203.1 NM_001282204.1 NM_002861.4 XM_005256386.3 XM_005256387.3 XM_006722287.3 XM_017024910.1 XM_017024911.1 XM_017024912.1

Validation Results:

5%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00161098_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.