SiRNA Validation Data

siRNA ID:

120755

Gene ID:

Transcript(s):

NM_005173.3 NM_174953.2 NM_174954.2 NM_174955.2 NM_174956.2 NM_174957.2 NM_174958.2 XM_011523881.2 XM_011523882.2 XM_011523884.2 XM_011523885.1 XM_011523888.2 XM_011523889.1 XM_017024692.1 XM_017024693.1

Validation Results:

21%(+/-5% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00193090_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.