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SiRNA Validation Data

siRNA ID:

1386

Gene ID:

81602

Transcript(s):

NM_001193478.1 NM_030911.3 NR_036439.1 XM_006719871.3 XM_011535250.2 XM_011535251.1 XM_011535253.1 XM_017020778.1 XM_017020779.1 XM_017020780.1 XM_017020781.1 XM_017020782.1 XM_017020783.1 XM_017020784.1

Validation Results:

28%(+/-0% mRNA remaining)(HeLa cell line)

Validation Procedure:

The above cell line was plated in at least triplicate the day before the transfection procedure was started. siRNA was transfected at a final concentration of 100 nM. RNA was extracted from the cells 48 hours after transfection. mRNA levels were measured by real-time RT-PCR using a random primer mix for the RT priming, followed by real time PCR. Knockdown is expressed as the relative amount of target mRNA remaining compared to that of cells transfected with a "nonsense" or scrambled siRNA negative control. Validation results are shown for multiple independent experiments.