SiRNA Validation Data

siRNA ID:

261192

Gene ID:

Transcript(s):

NM_001160226.1 NM_001160258.1 NM_001160259.1 NM_016083.4 NM_033181.3 XM_006715330.3 XM_011535424.1 XM_011535425.1 XM_011535426.1 XM_011535428.1 XM_017010238.1 XM_017010239.1 XM_017010240.1

Validation Results:

9%(+/-8% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00275634_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.