SiRNA Validation Data

siRNA ID:

s11638

Gene ID:

5833

Transcript(s):

NM_001184917.2 NM_001256433.2 NM_001256434.2 NM_001256435.2 NM_001282203.1 NM_001282204.1 NM_002861.4 XM_005256386.3 XM_005256387.3 XM_006722287.3 XM_017024910.1 XM_017024911.1 XM_017024912.1

Validation Results:

14%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00161098_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.