s37
NM_001030006.1
NM_001282.2
XM_005257937.3
XM_005257938.2
XM_005257941.2
XM_011524448.1
XM_011524449.2
XM_011524450.1
XM_011524451.1
XM_011524452.1
XM_011524453.1
XM_011524454.1
XM_011524455.2
XM_017024284.1
XM_017024285.1
XM_017024286.1
XM_017024287.1
10%(+/-4% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00426694_m1]
HeLa cells were reverse transfected with 5 nM gene-specific Silencer® Select siRNA or 5 nM Silencer® Select Negative Control #2 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.