SiRNA Validation Data

siRNA ID:

213304

Gene ID:

Transcript(s):

NM_001278554.1 NM_001278555.1 NM_006357.3 NM_182678.2 XM_005246244.2 XM_011510492.2 XM_017003166.1 XM_017003167.1 NR_110506.1

Validation Results:

20%(+/-5% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00818954_mH]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.