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SiRNA Validation Data

siRNA ID:

s8757

Gene ID:

4257

Transcript(s):

NM_001260511.1 NM_001260512.1 NM_001267598.1 NM_020300.4 NM_145764.2 NM_145791.2 NM_145792.2 NR_048545.1 NR_048547.1 XM_006719079.1 XM_011520673.1 XM_011520674.2

Validation Results:

1%(+/-0% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00220393_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer^® Select siRNA or 5 nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.