SiRNA Validation Data

siRNA ID:

s5100

Gene ID:

2232

Transcript(s):

NM_001258012.3 NM_001258013.3 NM_001258014.3 NM_001258015.2 NM_001258016.3 NM_004110.5 NM_024417.4 NR_047576.2 XM_006721772.1 XM_011524527.1 XM_011524528.1 XM_011524529.1 XM_011524531.2 XM_011524532.2 XM_011524533.2

Validation Results:

16%(+/-6% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00244586_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.