SiRNA Validation Data



Gene ID:



NM_003872.2 NM_018534.3 NM_201264.1 NM_201266.1 NM_201267.1 NM_201279.1 XM_005246933.3 XM_005246934.3 XM_017005185.1 XM_017005186.1 XM_017005187.1 XM_017005188.1

Validation Results:

28%(+/-4% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00187290_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.