SiRNA Validation Data



Gene ID:



NM_001123226.1 NM_012123.3 NM_133645.2 XM_006715444.2 XM_006715445.2 XM_006715446.3 XM_011535723.1 XM_011535724.1 XM_011535725.2 XM_017010697.1 XM_017010698.1 XM_017010699.1 XM_017010700.1

Validation Results:

12%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00201667_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.