SiRNA Validation Data

siRNA ID:

93

Gene ID:

817

Transcript(s):

NM_001221.3 NM_001321566.1 NM_001321567.1 NM_001321568.1 NM_001321569.1 NM_001321570.1 NM_001321571.1 NM_001321572.1 NM_001321573.1 NM_001321574.1 NM_001321575.1 NM_001321576.1 NM_001321577.1 NM_001321578.1 NM_001321579.1 NM_001321581.1 NM_001321583.1 NM_001321584.1 NM_001321585.1 NM_001321586.1 NM_001321587.1 NM_001321588.1 NM_001321589.1 NM_001321590.1 NM_001321591.1 NM_172114.1 NM_172115.2 NM_172127.2 NM_172128.2 XM_005263253.3 XM_005263254.3 XM_006714330.2 XM_011532292.2 XM_017008672.1 XM_017008673.1 XM_017008674.1 XM_017008675.1 XM_017008676.1 XM_017008677.1 XM_017008678.1

Validation Results:

8%(+/-0% mRNA remaining)(HeLa cell line)

Validation Procedure:

The above cell line was plated in at least triplicate the day before the transfection procedure was started. siRNA was transfected at a final concentration of 100 nM. RNA was extracted from the cells 48 hours after transfection. mRNA levels were measured by real-time RT-PCR using a random primer mix for the RT priming, followed by real time PCR. Knockdown is expressed as the relative amount of target mRNA remaining compared to that of cells transfected with a "nonsense" or scrambled siRNA negative control. Validation results are shown for multiple independent experiments.