SiRNA Validation Data

siRNA ID:

s2299

Gene ID:

780

Transcript(s):

NM_001202521.1 NM_001202522.1 NM_001202523.1 NM_001297652.1 NM_001297653.1 NM_001297654.1 NM_001954.4 NM_013993.2 NM_013994.2 XM_006715185.2 XM_011514882.2 XM_011514883.1 XM_011514884.1 XM_011514885.1 XM_011514886.1 XM_011514887.1 XM_011514888.2 XM_017011268.1 XM_017011269.1

Validation Results:

8%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00233612_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.