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SiRNA Validation Data

siRNA ID:

44066

Gene ID:

23759

Transcript(s):

NM_001317996.1 NM_014337.3 NM_148175.2 NM_148176.2 XM_005261448.3 XM_011530041.2 XM_011530042.2 XM_011530043.2 XM_011530044.2 XM_011530046.2 XM_011530047.2 XM_011530049.2 XM_011530050.2 XM_011530051.2

Validation Results:

14%(+/-10% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00204962_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.