SiRNA Validation Data



Gene ID:



NM_021003.4 NM_177951.2 NM_177952.2 XM_005267779.1 XM_005267781.1 XM_011536872.1 XM_011536876.1 XM_011536877.1 XM_011536878.1 XM_011536879.2 XM_011536880.2 XM_011536881.2 XM_011536882.2 XM_011536883.1 XM_011536884.1 XM_017021381.1 XM_017021382.1 XM_017021383.1 XM_017021384.1 XM_017021385.1 XM_017021386.1 XM_017021387.1

Validation Results:

11%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00221372_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.