SiRNA Validation Data



Gene ID:



NM_001003794.2 NM_001256585.1 NM_007283.6 XM_011512377.1 XM_011512378.1 XM_011512379.2 XM_011512383.2 XM_017005662.1 XM_017005663.1 XM_017005664.1 XM_017005666.1

Validation Results:

5%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00200752_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.