SiRNA Validation Data



Gene ID:



NM_003681.4 XM_005261195.2 XM_005261196.3 XM_005261198.2 XM_005261199.2 XM_011529758.2 XM_011529760.2 XM_011529761.1 XM_017028483.1 XM_017028484.1

Validation Results:

7%(+/-0% mRNA remaining)(HeLa cell line)

Validation Procedure:

The above cell line was plated in at least triplicate the day before the transfection procedure was started. siRNA was transfected at a final concentration of 100 nM. RNA was extracted from the cells 48 hours after transfection. mRNA levels were measured by real-time RT-PCR using a random primer mix for the RT priming, followed by real time PCR. Knockdown is expressed as the relative amount of target mRNA remaining compared to that of cells transfected with a "nonsense" or scrambled siRNA negative control. Validation results are shown for multiple independent experiments.