SiRNA Validation Data



Gene ID:



NM_003681.4 XM_005261195.2 XM_005261196.3 XM_005261198.2 XM_005261199.2 XM_011529758.2 XM_011529760.2 XM_011529761.1 XM_017028483.1 XM_017028484.1

Validation Results:

8%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00177600_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer® Select siRNA or 5 nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.