SiRNA Validation Data



Gene ID:



NM_001199261.1 NM_001199262.1 NM_001199263.1 NM_015984.3 NR_037607.1 XM_005245244.4 XM_005245246.4 XM_005245247.4 XM_005245248.4 XM_005245249.4 XM_006711366.3 XM_006711367.3 XM_006711368.3 XM_006711369.2 XM_006711370.3 XM_006711371.3 XM_011509604.1 XM_011509607.2 XM_011509608.2 XM_011509609.2 XM_017001429.1 XM_017001430.1 XM_017001431.1 XM_017001432.1 XM_017001433.1 XM_017001434.1 XM_017001435.1 XM_017001436.1 XM_017001437.1 XM_017001438.1 XM_017001439.1 XM_017001440.1 XM_017001441.1 XM_017001442.1 XM_017001443.1 XM_017001444.1

Validation Results:

7%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00255374_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.