SiRNA Validation Data

siRNA ID:

s506

Gene ID:

8500

Transcript(s):

NM_003626.3 NM_177423.2 NR_045286.1 XM_006718716.3 XM_011545306.2 XM_011545307.2 XM_011545308.2 XM_011545309.2 XM_011545310.2 XM_011545311.2 XM_011545312.2 XM_011545313.2 XM_011545314.2 XM_011545315.2 XM_011545316.2 XM_011545317.2 XM_011545318.2 XM_011545319.1 XM_017018448.1 XM_017018449.1 XM_017018450.1

Validation Results:

16%(+/-3% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00170349_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer® Select siRNA or 5 nM Silencer® Select Negative Control #2 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.