SiRNA Validation Data

siRNA ID:

107928

Gene ID:

Transcript(s):

NM_006037.3 XM_006712877.3 XM_006712878.3 XM_006712879.3 XM_006712880.3 XM_011512217.2 XM_011512218.2 XM_011512219.2 XM_011512220.2 XM_011512221.1 XM_011512222.2 XM_011512223.2 XM_011512224.2 XM_011512225.2 XM_011512226.2 XM_011512227.2 XM_011512230.1 XM_017005394.1 XM_017005395.1

Validation Results:

11%(+/-8% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00195814_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.