SiRNA Validation Data



Gene ID:



NM_001317851.1 NM_016173.4 XM_005265218.3 XM_011533806.2 XM_011533807.1 XM_011533808.1 XM_011533809.2 XM_011533810.2 XM_011533812.2 XM_011533813.2 XM_011533814.2

Validation Results:

20%(+/-3% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00275076_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.