SiRNA Validation Data

siRNA ID:

107720

Gene ID:

Transcript(s):

NM_001015053.1 NM_005474.4 XM_005256905.1 XM_005256906.4 XM_011524149.1 XM_011524150.1 XM_017023988.1 XM_017023989.1 XM_017023990.1 XM_017023991.1 XM_017023992.1 XM_017023993.1 XM_017023994.1 XM_017023995.1 XM_017023996.1

Validation Results:

28%(+/-7% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00608357_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.