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SiRNA Validation Data

siRNA ID:

s10221

Gene ID:

5151

Transcript(s):

NM_001243137.1 NM_002605.2 NM_173454.1 XM_011521668.2 XM_017022308.1 XM_017022309.1 XM_017022310.1 XM_017022311.1

Validation Results:

19%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00400174_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer^® Select siRNA or 5nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan^® Gene Expression Cells-to-CT^™ Kit and appropriate TaqMan^® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.