SiRNA Validation Data

siRNA ID:

s19459

Gene ID:

10013

Transcript(s):

NM_001321225.1 NM_001321226.1 NM_001321227.1 NM_001321228.1 NM_001321229.1 NM_006044.3 NR_135591.1 XM_017029187.1

Validation Results:

14%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00195869_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.