SiRNA Validation Data

siRNA ID:

121249

Gene ID:

Transcript(s):

NM_000234.2 NM_001289063.1 NM_001289064.1 NM_001320970.1 NM_001320971.1 NR_110296.1 NR_135497.1 NR_135498.1 NR_135499.1 NR_135500.1 NR_135501.1 XM_017026809.1

Validation Results:

6%(+/-4% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00172073_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.