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SiRNA Validation Data

siRNA ID:

1452

Gene ID:

5601

Transcript(s):

NM_001135044.1 NM_001308244.1 NM_002752.4 NM_139068.2 NM_139069.2 NM_139070.2 XM_006714891.2 XM_017009638.1 XM_017009641.1 XM_017009642.1 XM_017009643.1

Validation Results:

7%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00177102_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.