Close X

SiRNA Validation Data

siRNA ID:

s11159

Gene ID:

5601

Transcript(s):

NM_001135044.1 NM_001308244.1 NM_002752.4 NM_139068.2 NM_139069.2 NM_139070.2 XM_005265940.4 XM_006714891.2 XM_017009638.1 XM_017009639.1 XM_017009640.1 XM_017009641.1 XM_017009642.1 XM_017009643.1

Validation Results:

14%(+/-3% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00177102_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer^® Select siRNA or 5nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan^® Gene Expression Cells-to-CT^™ Kit and appropriate TaqMan^® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.