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SiRNA Validation Data

siRNA ID:

s5363

Gene ID:

2444

Transcript(s):

NM_002031.2 XM_005266880.4 XM_005266881.2 XM_005266882.4 XM_011535653.2 XM_011535654.2 XM_011535655.2 XM_017010645.1

Validation Results:

18%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00176619_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer^® Select siRNA or 5 nM Silencer^® Select Negative Control #2 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.