SiRNA Validation Data



Gene ID:



NM_001320977.1 NM_006122.3 NR_135502.1 NR_135503.1 NR_135504.1 XM_005254910.3 XM_011521565.2 XM_011521566.2 XM_017022184.1 XM_017022185.1

Validation Results:

16%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00196172_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.