SiRNA Validation Data

siRNA ID:

s13791

Gene ID:

6895

Transcript(s):

NM_004178.4 NM_134323.1 NM_134324.2 XM_005269114.1 XM_005269115.2 XM_005269117.1 XM_005269120.4 XM_005269122.3 XM_006719581.1 XM_011538712.2 XM_017019910.1 XM_017019911.1

Validation Results:

18%(+/-3% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00366328_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.