SiRNA Validation Data



Gene ID:



NM_001243027.2 NM_001243028.2 NM_001626.5 XM_011526614.1 XM_011526615.1 XM_011526616.1 XM_011526618.1 XM_011526619.1 XM_011526620.1 XM_011526622.2 XM_017026470.1 XM_017026471.1

Validation Results:

6%(+/-3% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00609846_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.