SiRNA Validation Data

siRNA ID:

103404

Gene ID:

Transcript(s):

NM_001031812.3 NM_001044723.2 NM_001270572.1 NM_001270573.1 NM_001270574.1 NM_004384.4 XM_005271891.1 XM_005271892.2 XM_005271894.2 XM_005271895.2 XM_005271896.1 XM_017009057.1 XM_017009058.1 XM_017009059.1 XM_017009060.1 XM_017009061.1 XM_017009062.1 XM_017009063.1 XM_017009064.1 XM_017009065.1 XM_017009066.1 XM_017009067.1 XM_017009068.1 XM_017009069.1 XM_017009070.1

Validation Results:

18%(+/-6% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00177858_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.