SiRNA Validation Data

siRNA ID:

105875

Gene ID:

Transcript(s):

NM_001002837.2 NM_001284285.1 NM_001284286.1 NM_001284287.1 NM_001284288.1 NM_001284289.1 XM_011530143.1 XM_017028771.1 XM_017028772.1 XM_017028773.1

Validation Results:

16%(+/-3% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00300155_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.