SiRNA Validation Data

siRNA ID:

106141

Gene ID:

7157

Transcript(s):

NM_000546.5 NM_001126112.2 NM_001126113.2 NM_001126114.2 NM_001126115.1 NM_001126116.1 NM_001126117.1 NM_001126118.1 NM_001276695.1 NM_001276696.1 NM_001276697.1 NM_001276698.1 NM_001276699.1 NM_001276760.1 NM_001276761.1

Validation Results:

10%(+/-5% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00153340_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.