SiRNA Validation Data

siRNA ID:

118352

Gene ID:

Transcript(s):

NM_000366.5 NM_001018004.1 NM_001018005.1 NM_001018006.1 NM_001018007.1 NM_001018008.1 NM_001018020.1 NM_001301244.1 NM_001301289.1 XM_005254637.1 XM_005254638.3 XM_005254639.3 XM_005254645.2 XM_005254646.2 XM_005254647.3 XM_005254650.3 XM_005254651.1 XM_005254652.1 XM_005254653.2 XM_006720667.3 XM_017022534.1 XM_017022535.1 XM_017022536.1 XM_017022537.1 XM_017022538.1 XM_017022539.1 XM_017022540.1 XM_017022541.1

Validation Results:

14%(+/-3% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00165966_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.