SiRNA Validation Data

siRNA ID:

270

Gene ID:

Transcript(s):

NM_001261816.1 NM_002613.4 NM_031268.5 XM_011522521.2 XM_011522523.2 XM_017023271.1 XM_017023272.1 XM_017023273.1 XM_017023274.1 XM_017023275.1 XM_017023276.1 XM_017023277.1

Validation Results:

28%(+/-6% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00176884_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.