SiRNA Validation Data

siRNA ID:

40536

Gene ID:

Transcript(s):

NM_001204492.1 NM_001222.3 NM_001320898.1 NM_172169.2 NM_172170.4 NM_172171.2 NM_172173.2 XM_005270195.1 XM_005270197.1 XM_005270198.1 XM_005270199.1 XM_005270200.1 XM_005270201.1 XM_005270203.1 XM_005270205.1 XM_006717993.1 XM_006717996.1 XM_006717997.1 XM_006717998.1 XM_017016716.1 XM_017016718.1 XM_017016719.1 XM_017016720.1 XM_017016721.1 XM_017016722.1 XM_017016723.1 XM_017016724.1 XM_017016725.1 XM_017016726.1 XM_017016727.1 XM_017016730.1 XM_017016731.1 XM_017016732.1 XM_017016734.1 XM_017016735.1 XM_017016736.1 XM_017016737.1 XM_017016738.1 XM_017016739.1

Validation Results:

12%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00299857_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.