SiRNA Validation Data



Gene ID:

834 440067


NM_001223.4 NM_001257118.2 NM_001257119.2 NM_033292.3 NM_033293.3 NM_033294.3 NM_033295.3 XM_017018393.1 XM_017018394.1 XM_017018395.1 XM_017018396.1 NR_131905.1

Validation Results:

5%(+/-3% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00169146_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.