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SiRNA Validation Data

siRNA ID:

s11077

Gene ID:

5570

Transcript(s):

NM_001270393.1 NM_001270394.1 NM_001270395.1 NM_032471.5 NM_181794.2 NM_181795.2 XM_011535930.2 XM_011535931.2 XM_011535932.2 XM_011535933.2 XM_011535934.2 XM_011535935.2 XM_011535936.1 XM_011535937.2

Validation Results:

4%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00261162_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer^® Select siRNA or 5nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan^® Gene Expression Cells-to-CT^™ Kit and appropriate TaqMan^® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.